Cryo-EM Sample Fate — a Schematic Model

Cross-section of the vitreous ice film on a grid; each glowing dot is one protein molecule. Illustrative only — not a predictor of experimental outcomes.

Simulated orientation coverage

 

Adsorbed (model)0
Lost at interface0
View directions0/12
diffusing interface-adsorbed unfolded support-adsorbed
Schematic teaching model of behaviors described in the cited literature — particle counts and probabilities are illustrative, not measured kinetics or performance predictions. Real outcomes are specimen-dependent and vary with buffer, functionalization chemistry, transfer quality, and blotting conditions.